Abstract: Dissolved organic matter (DOM) had different migration and transformation characteristics with inor-ganic components in the process of groundwater movement,and there were obvious differences in the concentration,type and fluorescence intensity at different aquifers. Therefore,the authors studied the determination method of mine water in-rush sources with the combination of organic-inorganic water chemistry. The result showed that the distribution of inor-ganic matter concentration in groundwater had vertical zoning,which could distinguish the difference of hydrochemical characteristics between shallow aquifers and deep aquifers. Based on the hydrogeo-logic condition and the law of hydro-geochemical occurrence in the study area,the hydro-chemical anomaly samples could be distinguished using pH,mineralizing degree establishment of water chemical characteristics standard in different aquifers. DOM had a strong oxidation-reduction re-action and its concentration (i. e. TOC and UV254) changed rapidly after entering the aquifer,which could be used to distinguish the hydrochemical characteristics of the shallow aquifers,such as the Quaternary and Cretaceous aquifers. The concentrations of the inorganic and organic components in the aquifers were very similar,but the fluorescence fin-gerprint had high sensitivity in the destruct height of overlying strata. The water source of each aquifer could be deter-mined by the analysis of the difference of DOM type and fluorescence peak intensity according to 3DEEM spectro-gram. The geological anomaly bodies such as the collapse column were special geological environment bodies,in which DOM was relatively rich. By analyzing the DOM concentration and the characteristics of the fluorescence fingerprint, the difference of the hydrochemical characteristics with the Ordovician limestone aquifer could be distinguished well. Inorganic components had vertical zoning and could discriminate hydrochemical anomalies. The water sources of differ-ent aquifers could be determined by the analysis of chemical characteristics of organic and inorganic components, which could provide a scientific basis for quickly determining water sources when mine water inrush accidents occur.(TDS),HCO3 ,SO4 and other inorganic indexes,which could provide a scientific basis for the