Abstract: Inhibiting oxidative spontaneous combustion of high sulfur coals for green economy and environmental friendliness. Desulphurization using sustainable, low-energy microbiological methods. Gloeophyllum trabeum is selected to lixiviate high sulfur coal. Gloeophyllum trabeum reacts with Fenton reaction in the degradation of cellulose. and its product H₂O₂ undergoes oxidation-reduction reaction with coal to remove FeS₂, to inhibit coal spontaneous combustion. The slurry was reacted by Gloeophyllum trabeum for 30 days, after which it was acid washed and dried, sulfur determination, proximate analysis, X-ray diffraction, Fourier transform infrared spectroscopy, simultaneous TG-DSC apparatus and other experiments. From the macroscopic characteristics and microscopic mechanism, clarify the coal structure and composition variation, after the action of Gloeophyllum trabeum. The results show that the desulfurization rate of the high sulfur coals after the interaction of Gloeophyllum trabeum is above 50%, mainly removing inorganic sulfur, and the volatile matter is decreased after desulfurization. X-ray diffraction results are analyzed by Jade software. The weight percentage of FeS₂ decreases from 64.30% to 52.40% and from 57.10% to 45.70% in both coal samples, and the weight percentage of quartz increases by Gloeophyllum trabeum. High-sulfur coals with a sulfur content of 5.02% have an elevated aromatic hydrocarbon content of 2.81% and a decreased oxygenated functional group content of 2.38%; the high sulfur coal with 3.04% sulfur content has an elevated aromatic hydrocarbon content of 3.79%, a decreased oxygenated functional group content of 4.23%, and a decreased aliphatic hydrocarbon content of 1.92%. The characteristic temperature point of the coal is delayed after desulfurization, in which the ignition point T₆ of the two coal samples is delayed by 18 ℃ and 32 ℃, and the total heat release of the two coal samples is reduced by 472.66 J/g and 348.19 J/g; The activation energy E in the thermal decomposition stage is increased by 11.79 kJ/mol and 38.67 kJ/mol and finger front factor A become larger, indicating that coal oxidation reactions are difficult to occur after desulfurization with Gloeophyllum trabeum. So it is feasible for Gloeophyllum trabeum to desulfurize and inhibit the coal spontaneous combustion, which provides an experimental foundation for the inhibition of high sulfur coal oxidation.