煤矿沉陷区表层土壤细菌群落固碳固氮功能对水蚀强度的响应

Response of carbon and nitrogen sequestration capacities of surface soil microbial community to hydraulic erosion in coal mine subsidence areas

  • 摘要: 煤矿沉陷区水力侵蚀(水蚀)前后,表层土壤细菌群落的多样性、结构及其固碳固氮功能的变化已受到关注。然而,针对不同水蚀强度下细菌群落特征演变规律的研究尚不系统。为此,系统采集了煤矿沉陷区34组不同水蚀强度下的表层土壤样品,并采用高通量测序、结构方程模型及分子生态网络分析等技术手段,探索了轻度、中度和重度水蚀强度下表层土壤细菌群落特征、组装机制及固碳固氮功能的演变规律。结果表明:与未水蚀区域相比,轻度、中度和重度水蚀强度下,煤矿沉陷区表层土壤有机碳分别下降了33.57%、69.66%和82.63%,而总氮质量分数减少了57.02%、71.33%和85.28%。于此同时,细菌群落的Sobs指数下降了18.82%、23.17%和36.60%,ACE指数也随之下降了22.96%、25.83%和36.52%。虽然不同水蚀强度下煤矿沉陷区表层土壤细菌群落的构建模式仍以随机性过程为主导,但随着水蚀强度的增强,随机性作用逐渐减弱,而确定性作用则逐渐增强。表层土壤中固碳固氮细菌群落与环境因子之间的关联性逐渐加强,而细菌群落间的协作性、信息传递效率以及能量流动速率则逐渐减缓。研究结果为揭示煤矿沉陷区水蚀过程中表层土壤微生物群落与碳氮循环相互作用机制提供了新的科学依据。

     

    Abstract: The different in surface soil microbial diversity, community structure, and the potential for carbon and nitrogen sequestration before and after hydraulic erosion in coal mine subsidence areas have been extensively investigated. However, the dynamic evolution characteristics under varying intensities of hydraulic erosion remain poorly understood. To address this knowledge gap, 34 groups of surface soil samples were systematically collected from coal mine subsidence areas subjected to different hydraulic erosion intensities. High-throughput sequencing, structural equation modeling, and molecular ecological network analysis were employed to elucidate the characteristics, assembly mechanisms, and evolutionary patterns of bacterial communities in surface soils under mild, moderate, and severe hydraulic erosion conditions. The findings reveal that, in comparison to non-water erosion areas, soil organic carbon in coal mine subsidence areas decreased by 33.57%, 69.66%, and 82.63% under light, moderate, and severe hydraulic erosion intensities, respectively. Similarly, total nitrogen content decreased by 57.02%, 71.33%, and 85.28%. Concurrently, the Sobs index of the bacterial community exhibited reductions of 18.82%, 23.17%, and 36.60%, while the ACE index also showed declines of 22.96%, 25.83%, and 36.52%. Although random processes predominantly govern the surface soil bacterial community assembly model, the influence of random factors decreased while deterministic factors become more significant with increasing hydraulic erosion intensity. Additionally, the relationship between surface soil bacterial communities and environmental factors became increasingly intimate, whereas the rates of cooperation, information exchange, and energy transfer among bacteria gradually decelerated. These results offer novel insights into the interactions between surface soil microorganisms and carbon and nitrogen dynamics during hydraulic erosion process.

     

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