Abstract: The permeable reactive barrier based on sulfate-reducing bacteria biocathodes MFC ( SRB-PRB) was used in the in-situ treatment of acid mine water,and MFC structure and its layout design on SRB-PRB,pH value,ratio of C / S and heavy metal ion concentration are its main influences for pre-condition capacity. Two MFC structure ( cylin- drical,flat) and three different layouts ( lattice,downstream,lateral flow) in SRB-PRB were conducted for the same volume reactive medias in the PRB for the investigation of AMD pre-condition efficiency under the different MFC structure. The effluent concentration of Fe2+ ,Cu2+ and Zn2+ were 30 mg / L under the AMD conditions:pH = 3 and ρ(SO24- )= 3 000 mg / L. Experimental results show that the effects of MFC on precondition is associated with the avail- ability of sludge carbon source,the MFC structure and the PRB layout,the influence degree are as follows:carbon source>MFC structure>layout. Cylindrical MFC has a better performance than flat MFC,the maximum power densities is twice of flat MFC. The pH values are higher than the flat type,and is kept at about 7. 0 stably. The percent removal of Fe2+ ,Cu2+ and Zn2+ are 96. 2% -100% ,99. 6% -100% ,95. 3% -99. 3% respectively. The average removal of SO24- of cylindrical MFC is 7 g / (m3·h) higher than flat type. Lattice layout in SRB-PRB presents a characterizes of stable performance and flexible design.