Abstract: In order to understand the organic pollution characteristics of mine water in different position of underground coal mine, one coal mine was used as an object of study in Shenfu mining area.The water quality characteristics of mine water were researched based on three dimensional fluorescence fingerprint technology and fluorescence regional integration.The results showed that the cations and anions in mine water were from coal seam roof aquifers.Some pollutants (e.g.nitrogen, organic) were from underground coal mining.The exceeding standard rate of COD was 72.7% more than third level standard for groundwater quality.The COD concentrations of 02 working face mine water were up to fifth level standard for groundwater quality, and TOC and UV₂₅₄ were also high.There were aromatic protein fluorescent peaks in Mine water (No.1-5).Region V fluorescent peaks were very obvious in working face mine water, and the organic matter was from fully mechanized mining equipment oil during coal mining and equipment maintaining.The sum of P_{i, n} and P_{i, n} of each mine water (No.1-5) was more than 52.0% of P_{i, n}, which showed that aromatic protein was very important DOM in groundwater of this area.The P_{i, n} of polycyclic aromatic hydrocarbons increased obviously in mine water of 02 working face, which showed that large quantities of oil leaked from fully mechanized mining equipment to mine water.The water quality of inlet water and outlet water of mine water treatment plant had comprehensive characteristics of mine water in different position of underground coal mine, and the organic matters of region Ⅰ and region Ⅴ could be utilized by microbes and removed by mine water treatment processes.The low correlations between Φ_i, P_{i, n}, FI, TOC, UV₂₅₄ indicated that undissolved slime was main component of COD.While there were high correlations between UV₂₅₄ and TOC and Φ_i, P_{i, n}, FI, which showed that FRI and peak-picking technique could characterize the fluorescence characteristics and contents of DOM.